Gene description for RAC1
Gene name	Rac family small GTPase 1
Gene symbol	RAC1
Other names/aliases	MIG5 MRD48 Rac-1 TC-25 p21-Rac1
Species	Homo sapiens
Experiment description of studies that identified RAC1 in Glioblastoma cells
1 Experiment ID 1072 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 29937354    Organism Homo sapiens Experiment description Apoptotic Cell-Derived Extracellular Vesicles Promote Malignancy of Glioblastoma Via Intercellular Transfer of Splicing Factors. Authors Pavlyukov MS, Yu H, Bastola S, Minata M, Shender VO, Lee Y, Zhang S, Wang J, Komarova S, Wang J, Yamaguchi S, Alsheikh HA, Shi J, Chen D, Mohyeldin A, Kim SH, Shin YJ, Anufrieva K, Evtushenko EG, Antipova NV, Arapidi GP, Govorun V, Pestov NB, Shakhparonov MI, Lee LJ, Nam DH, Nakano I. Journal name Cancer Cell Publication year 2018 Sample Glioblastoma cells Sample name Lethally irradiated GBM157 - apoExo - biorep2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
2 Experiment ID 1242 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 25261722    Organism Homo sapiens Experiment description Inflammatory cytokines, interleukin-1 beta and tumor necrosis factor-alpha, upregulated in glioblastoma multiforme, raise the levels of CRYAB in exosomes secreted by U373 glioma cells. Authors Kore RA, Abraham EC. Journal name Biochem Biophys Res Commun. Publication year 2014 Sample Glioblastoma cells Sample name U373 cells treated with 10ng/ml of IL-1. Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140183: EV-METRIC:22%
3 Experiment ID 1243 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 25261722    Organism Homo sapiens Experiment description Inflammatory cytokines, interleukin-1 beta and tumor necrosis factor-alpha, upregulated in glioblastoma multiforme, raise the levels of CRYAB in exosomes secreted by U373 glioma cells. Authors Kore RA, Abraham EC. Journal name Biochem Biophys Res Commun. Publication year 2014 Sample Glioblastoma cells Sample name U373 cells treated with 10ng/ml of TNF-. Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140183: EV-METRIC:22%
4 Experiment ID 1274 Identified molecule protein Extracellular vesicle type Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23807490    Organism Homo sapiens Experiment description Glioma microvesicles carry selectively packaged coding and non-coding RNAs which alter gene expression in recipient cells. Authors Li CC, Eaton SA, Young PE, Lee M, Shuttleworth R, Humphreys DT, Grau GE, Combes V, Bebawy M, Gong J, Brammah S, Buckland ME, Suter CM. Journal name RNA Biol. Publication year 2013 Sample Glioblastoma cells Sample name U251 cells Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Mass spectrometry Microarray EV-TRACK EV130098: EV-METRIC:29%